Analytical Biochemistry: Alert 10 April-16 April

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Alert: Analytical Biochemistry

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Analytical Biochemistry Volume 476 ,  Pages 1-94, 1 May 2015 Methods in the Biological Sciences, submit your next methods paper today. Editorial Board    Pages ii-iii   Regular Articles Positive selection improves the efficiency of DNA assembly   Original Research Article Pages 1-4 Chang-Ho Baek, Jonathan Chesnut, Federico Katzen Temperature manifold for a stopped-flow machine to allow measurements from −10 to +40 °C   Original Research Article Pages 11-16 Jonathan Walklate, Michael A. Geeves Selective oxidation of enzyme extracts for improved quantification of peroxidase activity   Original Research Article Pages 20-25 Shu Jiang, Michael H. Penner Sandwich enzyme-linked immunosorbent assay for the quantification of human serum albumin fragment 408–423 in bodily fluids   Original Research Article Pages 29-35 Katharina B. Mohr, Onofrio Zirafi, Mark Hennies, Sebastian Wiese, Frank Kirchhoff, Jan Munch Differentiating between monozygotic twins through DNA methylation-specific high-resolution melt curve analysis   Original Research Article Pages 36-39 Leander Stewart, Neil Evans, Kimberley J. Bexon, Dieudonne J. van der Meer, Graham A. Williams A single-vector EYFP reporter gene assay for G protein-coupled receptors   Original Research Article Pages 40-44 Helle Hald, Boqian Wu, Lamine Bouakaz, Morten Meldal A high-throughput mass spectrometric assay for discovery of human lipoxygenase inhibitors and allosteric effectors   Original Research Article Pages 45-50 J. Brian Jameson, Victor Kenyon, Theodore R. Holman A fluorescence spectroscopy assay for real-time monitoring of enzyme immobilization into mesoporous silica particles   Original Research Article Pages 51-58 Pegah S. Nabavi Zadeh, Kassam Abdel Mallak, Nils Carlsson, Bjorn Akerman Coupling purification and in situ immobilization process of monoclonal antibodies to clenbuterol for immunosensor application   Original Research Article Pages 59-66 Hui Cao, Min Yuan, Lili Wang, Jingsong Yu, Fei Xu Measurement of 15N enrichment of glutamine and urea cycle amino acids derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using liquid chromatography–tandem quadrupole mass spectrometry   Original Research Article Pages 67-77 Hidehiro Nakamura, Sachise Karakawa, Akiko Watanabe, Yasuko Kawamata, Tomomi Kuwahara, Kazutaka Shimbo, Ryosei Sakai Indirect purification method provides high yield and quality ssDNA sublibrary for potential aptamer selection   Original Research Article Pages 84-90 Yinze Zhang, Hua Xu, Huayou Zhou, Fan Wu, Yuqin Su, Yanlian Liang, Dan Zhou   Notes And Tips Immunofluorescent detection of the activation of the small GTPase Rac1 in mouse skeletal muscle fibers    Pages 5-7 Nobuyuki Takenaka, Yuma Nihata, Takaya Satoh Comparison of eight commercially available kits for DNA extraction from formalin-fixed paraffin-embedded tissues    Pages 8-10 Anna Janecka, Agnieszka Adamczyk, Anna Gasinska Evaluation of SYPRO Ruby total protein stain for the normalization of two-dimensional Western blots    Pages 17-19 Birgit Steinberger, Gottfried Brem, Corina Mayrhofer An efficient method for multiple site-directed mutagenesis using type IIs restriction enzymes    Pages 26-28 Zhiqiang Zhang, Kun Xu, Ying Xin, Zhiying Zhang A fluorescence resonance energy transfer-based method for histone methyltransferases    Pages 78-80 Kanchan Devkota, Brian Lohse, Camilla Nyby Jakobsen, Jens Berthelsen, Rasmus Prætorius Clausen High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-l-methionine    Pages 81-83 Jianyu Zhang, Judith P. Klinman Seed oil polyphenols: Rapid and sensitive extraction method and high resolution–mass spectrometry identification    Pages 91-93 Mohamed Koubaa, Houcine Mhemdi, Eugene Vorobiev Analytical Biochemistry Volume 477 ,  Pages 1-118, 15 May 2015 Editorial Board    Pages ii-iii   Regular Articles Nine surface plasmon resonance assays for specific protein quantitation during cell culture and process development   Original Research Article Pages 1-9 Asa Frostell, Anna Mattsson, Asa Eriksson, Elisabeth Wallby, Johan Karnhall, Nina B. Illarionova, Camilla Estmer Nilsson Routine Western blot to check autophagic flux: Cautions and recommendations   Original Research Article Pages 13-20 Ruben Gomez-Sanchez, Elisa Pizarro-Estrella, Sokhna M.S. Yakhine-Diop, Mario Rodriguez-Arribas, Jose M. Bravo-San Pedro, Jose M. Fuentes, Rosa A. Gonzalez-Polo Immobilized trypsin on hydrophobic cellulose decorated nanoparticles shows good stability and reusability for protein digestion   Original Research Article Pages 21-27 Xiuxia Sun, Xiangdong Cai, Ren-Qi Wang, Jianxi Xiao A novel label-free upconversion fluorescence resonance energy transfer-nanosensor for ultrasensitive detection of protamine and heparin   Original Research Article Pages 28-34 Qian Long, Jiangna Zhao, Bangda Yin, Haitao Li, Youyu Zhang, Shouzhuo Yao mTRAQ-based quantitative analysis combined with peptide fractionation based on cysteinyl peptide enrichment   Original Research Article Pages 41-49 Jeonghun Yeom, Min Jung Kang, Dongyun Shin, Hyun Kyu Song, Cheolju Lee, Ji Eun Lee Improved Fab presentation on phage surface with the use of molecular chaperone coplasmid system   Original Research Article Pages 56-61 Qiuting Loh, Siew Wen Leong, Gee Jun Tye, Yee Siew Choong, Theam Soon Lim 19F nuclear magnetic resonance screening of glucokinase activators   Original Research Article Pages 62-68 O. Assemat, M. Antoine, J.-M. Fourquez, M. Wierzbicki, Y. Charton, P. Hennig, F. Perron-Sierra, G. Ferry, J.A. Boutin, M.-A. Delsuc Fluorogenic DNA ligase and base excision repair enzyme assays using substrates labeled with single fluorophores   Original Research Article Pages 69-77 Theo T. Nikiforov, Steven Roman A quantitative lateral flow assay to detect complement activation in blood   Original Research Article Pages 78-85 Elizabeth C. Schramm, Nick R. Staten, Zhouning Zhang, Samuel S. Bruce, Christopher Kellner, John P. Atkinson, Vasileios C. Kyttaris, George C. Tsokos, Michelle Petri, E. Sander Connolly, Paul K. Olson Analysis of inter-/intra-E-plate repeatability in the real-time cell analyzer   Original Research Article Pages 98-104 Jiao Chen, Tianhong Pan, Bharathi Devi Devendran, Zhankun Xi, Swanand Khare, Biao Huang, Weiping Zhang, Stephan Gabos, Dorothy Yu Huang, Can Jin The relations between metabolic variations and genetic evolution of different species   Original Research Article Pages 105-114 Zhishui Li, Chenghong Lin, Jingjing Xu, Huifeng Wu, Jianghua Feng, Heguang Huang   Notes And Tips Preparation of ready-to-use small unilamellar phospholipid vesicles by ultrasonication with a beaker resonator    Pages 10-12 Johannes Klingler, Carolyn Vargas, Sebastian Fiedler, Sandro Keller Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems    Pages 35-37 Anna Glyk, Sandra L. Heinisch, Thomas Scheper, Sascha Beutel Increased transcriptome sequencing efficiency with modified Mint-2 digestion–ligation protocol    Pages 38-40 Juhana I. Kammonen, Olli-Pekka Smolander, Timo Sipila, Kirk Overmyer, Petri Auvinen, Lars Paulin A monophasic solution for isolation of RNA devoid of polymerase chain reaction-detectable genomic DNA contamination    Pages 50-52 Ujjal Ghosh, Shankhamala Bose, Trinath Ghosh, Tapas Kumar Bandyopadhyay, Utpal Basu Tracking a protein following dissociation from a protein–protein complex using a split SNAP-tag system    Pages 53-55 Masayasu Mie, Tatsuhiko Naoki, Eiry Kobatake Using photosystem I as a reporter protein for 13C analysis in a coculture containing cyanobacterium and a heterotrophic bacterium    Pages 86-88 Le You, Haijun Liu, Robert E. Blankenship, Yinjie J. Tang Hydroxamate-based colorimetric assay to assess amide bond formation by adenylation domain of nonribosomal peptide synthetases    Pages 89-91 Ryotaro Hara, Ryohei Suzuki, Kuniki Kino Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes    Pages 92-94 Peter C. Fridy, Mary K. Thompson, Natalia E. Ketaren, Michael P. Rout Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins    Pages 95-97 Yuxuan Xiao, Daniel Pollack, Edward Nieves, Aby Winchell, Myrasol Callaway, Margarita Vigodner Choice of PCR microtube can impact on the success of long-range PCRs    Pages 115-117 Eng-Wee Chua, Allison L. Miller, Martin A. Kennedy

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